| Cartoon | The Dark, Clenched Hole Of Evil |
| Ed Helms | ... | Half-Scary |
| Steve Carell | ... | Bighead |
| Steve Carell | ... | commissioner Voice |
| Steve Carell | ... | Gary Voice |
| Stephen Colbert | ... | Dr. Brainio |
| Stephen Colbert | ... | Ace Voice |
| Stephen Colbert | ... | Dr. Brainio Voice |
| Jimmy Fallon | ... | Gary |
| Jon Hamm | ... | Ace |
| Bill Chott | ... | announcer Voice |
| Fred Armisen | ... | Lizardo |
| Robert Smigel | ... | Bighead Voice |
| Robert Smigel | ... | Half-Scary Voice |
| Robert Smigel | ... | Lizardo Voice |
| Shawn Michael | ... | henchman |